ProCode™ Science

The Protein Transport System

Vybion technology takes advantage of the twin arginine transport(TAT) system.

  • Transports only folded proteins from the cytoplasm to the periplasmic space in E. coli
  • Antibiotic reporter system is cloned 3' and used to select clones from a library of sequence changes

  • Only properly folded proteins are transported and thus sensitive to antibiotic.
  • Strength of antibiotic can also be adjusted to increase the stringency of selection criteria.
  • Selection by growth on antibiotic is followed by colony PCR to identify specific sequence changes.

 

Antibody Selection Tools

  • Accommodates more complex proteins than would otherwise be expressed in E. coli
  • Single chain Fv selection enables identification of a larger, more diverse pool of high-affinity antibodies
  • Proteins up to at least 140 kDa can be transported through the TAT pore
  • Easily accommodates interacting polypeptides up to 45 kDa each comprising an scFv and antigen complex.