ProCode Intrabody Selection Overview:

Next-generation scFv platform in bacteria: Novel quality control selection eliminates scFv aggregation in bacterial systems.

Key advantages

  • Proprietary libraries (filter out stops, control expression levels and eliminate aggregation)
  • ProCode scFvs are engineered as vectorized Intrabodies (AAV delivery)
    • Validated for intracellular targets
    • Intrabody based drugs cut across all therapeutic areas
  • Only direct Intrabody platform
  • Compatible vectors for multiple formats: scFv, bivalent and bispecific miniMab formats (scFvs to IgG cassettes)

Selected References:

  1. Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli. Lim HK, Mansell TJ, Linderman SW, Fisher AC, Dyson MR, Delisa MP. Protein Sci. 2009 Oct 14. [Epub ahead of print]PMID:19830686
  2. Discovery of amyloid-beta aggregation inhibitors using an engineered assay for intracellular protein folding and solubility. Lee LL, Ha H, Chang YT, DeLisa MP. Protein Sci. 2009 Feb;18(2):277-86.PMID: 19177561
  3. Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism. Waraho D, DeLisa MP. Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3692-7. Epub 2009 Feb 20.PMID: 19234130
  4. Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery. Fisher AC, DeLisa MP. J Mol Biol. 2009 Jan 9;385(1):299-311. Epub 2008 Nov 1.PMID: 18992254
  5. Following the path of a twin-arginine precursor along the TatABC translocase of Escherichia coli. Panahandeh S, Maurer C, Moser M, DeLisa MP, Müller M. J Biol Chem. 2008 Nov 28;283(48):33267-75. Epub 2008 Oct 3.PMID: 18836181
  6. Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control. Fisher AC, DeLisa MP. PLoS One. 2008 Jun 11;3(6):e2351.PMID: 18545653
  7. Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway. Fisher AC, Kim W, DeLisa MP. Protein Sci. 2006 Mar;15(3):449-58. Epub 2006 Feb 1.PMID: 16452624
  8. Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli. Kim JY, Fogarty EA, Lu FJ, Zhu H, Wheelock GD, Henderson LA, DeLisa MP. Appl Environ Microbiol. 2005 Dec;71(12):8451-9.PMID: 16332834
  9. A little help from my friends: quality control of presecretory proteins in bacteria. Fisher AC, DeLisa MP. J Bacteriol. 2004 Nov;186(22):7467-73. Review. PMID: 15516557